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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Hello OME Team,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I am working on an image acquisition pipeline that writes to a tiled OME-TIFF. Let’s say for argument’s sake, my final image is 4096 x 4096. What I would like to do is:<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><ol style='margin-top:0in' start=1 type=1><li class=MsoListParagraph style='margin-left:0in;mso-list:l0 level1 lfo1'>Write the first region (2048 x 2048) I acquired to the top-left of the final image. I call <i>saveBytes</i> with a ROI defined as (0, 0, 2048, 2048) [x, y, width, height]. This works okay.<o:p></o:p></li><li class=MsoListParagraph style='margin-left:0in;mso-list:l0 level1 lfo1'>Write the second region (2048 x 2048) to the right of the first region. I call <i>saveBytes</i> with a ROI defined as (2048, 0, 2048, 2048). This does not get written.<o:p></o:p></li></ol><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I have also tried variations where I write to the bottom of the first region, which doesn’t work either. Is there a mechanism that allows me to sequentially write regions of interest to the same plane?<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Thanks,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><b>Dennis Ai</b><o:p></o:p></p><p class=MsoNormal><span style='font-size:9.0pt'>Senior Strategic Advisor, SRI Biosciences</span><o:p></o:p></p><p class=MsoNormal><span style='font-size:9.0pt'>SRI International</span><o:p></o:p></p><p class=MsoNormal><span style='font-size:9.0pt'>333 Ravenswood Ave</span><o:p></o:p></p><p class=MsoNormal><span style='font-size:9.0pt'>Menlo Park, CA 94025</span><o:p></o:p></p><p class=MsoNormal><span style='font-size:9.0pt'>(847) 440-4765</span><o:p></o:p></p><p class=MsoNormal> <o:p></o:p></p><p class=MsoNormal><b><span style='font-size:14.0pt;font-family:"Helvetica",sans-serif'>SRI </span></b><span style='font-size:14.0pt;font-family:"Helvetica",sans-serif'>Biosciences</span><sup><span style='font-size:10.0pt;font-family:"Helvetica",sans-serif'>™</span></sup><o:p></o:p></p><p class=MsoNormal><span style='font-size:6.0pt;font-family:"Helvetica",sans-serif'>A DIVISION OF SRI INTERNATIONAL</span><o:p></o:p></p><p class=MsoNormal> <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>