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Hi Manuel,
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<div class="">On 17 Jul 2017, at 15:32, Manuel Stritt <<a href="mailto:manuel.stritt@idorsia.com" class="">manuel.stritt@idorsia.com</a>> wrote:</div>
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<div class="">Dear all,</div>
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<div class="">I've been informed by the vendor that in BF images the white point (the gray values to be skipped for visualization) is stored by the machine and </div>
<div class="">it should be possible to read it via the CZIReader.</div>
<div class="">(Not sure if 'white balance' is the correct wording here.) </div>
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<div class="">So far I did not find a specific meta data for that. Do you know anything about this value and can tell me how to read it out?</div>
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<div>Looking at a couple of example CZI files, I would think the following key/value pairs in the original</div>
<div>metadata table populate at the reader initialisation would contain the information you need:</div>
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</span><span class="">$ ~/bftools-5.5.3/showinf -nopix 2015_08_21__16_09__0954.czi | grep WhiteBalance<br class="">
</span><span class="">Experiment|AcquisitionBlock|AcquisitionModeSetup|Camera|WhiteBalance #1: 0.40944686621105,0.50887655000016,1<br class="">
Experiment|AcquisitionBlock|AcquisitionModeSetup|Camera|WhiteBalanceOffset #1: 0<br class="">
Experiment|AcquisitionBlock|AcquisitionModeSetup|Camera|WhiteBalanceOffset|Status #1: Valid<br class="">
Experiment|AcquisitionBlock|AcquisitionModeSetup|Camera|WhiteBalance|Status #1: Valid<br class="">
HardwareSetting|ParameterCollection|WhiteBalance #1: 0.55201666149549,1,0.79661143765063</span></div>
<span class="">HardwareSetting|ParameterCollection|WhiteBalance #2: 0.40944686621105,0.50887655000016,1<br class="">
HardwareSetting|ParameterCollection|WhiteBalance.Default #1: 1.01,0.8,0.5<br class="">
HardwareSetting|ParameterCollection|WhiteBalance.Default #2: 1,1,1<br class="">
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<div class="">Or maybe even more in general - is there a white balance meta data field even for other formats?</div>
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<div>It is certainly possible other formats store the same information. Note however that</div>
<div>this information will be available via the Original Metadata and not the OME metadata.</div>
<div>This means that both the existence and the nomenclature will be vendor specific with no</div>
<div>cross-format naming consistency [1].</div>
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<div>Best,</div>
<div>Sebastien</div>
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[1] <a href="https://www.openmicroscopy.org/site/support/bio-formats5.5/about/index.html#bio-formats-metadata-processing" class="">https://www.openmicroscopy.org/site/support/bio-formats5.5/about/index.html#bio-formats-metadata-processing</a> </div>
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<div class="">Thanks for your help!</div>
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<div class="">Regards,</div>
<div class="">Manuel</div>
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