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Hi Sebastien,</div>
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thanks very much for the prompt response and valuable pointers. With Lee’s workaround everything now looks as expected.</div>
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For now I am ok with slightly downgraded schema versions but should think about an automated updating scheme on the long run, I guess. But that’s for later.</div>
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Thanks</div>
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   Heinrich</div>
<br>
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<br>
<p class="airmail_on">On 27 February 2017 at 10:07:47, Sebastien Besson (Staff) (<a href="mailto:s.besson@dundee.ac.uk">s.besson@dundee.ac.uk</a>) wrote:</p>
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Hi Heinrich,
<div class=""><br class="">
</div>
<div class="">thanks for the additional information and the sample file. I confirm that the round-tripping in Fiji works</div>
<div class="">as expected preserving both the dimensionality and metadata.</div>
<div class=""><br class="">
</div>
<div class="">In the meantime, I realise I misunderstood that metadata you pasted in your original email was the output</div>
<div class="">of python-bioformats. This now makes sense and I think this explains where the issue comes from.</div>
<div class=""><br class="">
</div>
<div class="">By default, python-bioformats detects file formats using a different strategy than Image/Fiji. The following thread</div>
<div class="">by the CellProfiler team [1] explains the issue and the rationale behind their design. Effectively, this causes</div>
<div class="">OME-TIFF fillets to be detected as simple multi-page TIFFs with the dimensions are interpreted as a time-series.</div>
<div class="">The workaround suggested by Lee in this issue should allow you to create an ImageReader and open the files</div>
<div class="">with the same behavior as Fiji/ImageJ.</div>
<div class=""><br class="">
</div>
<div class="">As indicated by the 2015-01 schema version, note also that python-bioformats is still using Bio-Formats 5.1.x [2].</div>
<div class="">This means the metadata of your exported OME-TIFF using the 2016-06 schema version will effectively be</div>
<div class="">downgraded to the 2015-01 version of the OME model on read.</div>
<div class=""><br class="">
</div>
<div class="">Best,</div>
<div class="">Sebastien</div>
<div class=""><br class="">
</div>
<div class="">[1] <a href="https://github.com/CellProfiler/python-bioformats/issues/23" class="">https://github.com/CellProfiler/python-bioformats/issues/23</a></div>
<div class="">[2] <a href="https://github.com/CellProfiler/python-bioformats/issues/62" class="">https://github.com/CellProfiler/python-bioformats/issues/62</a> </div>
<div class=""><br class="">
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<div class="">
<div>
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<div class="">On 24 Feb 2017, at 18:00, Grabmayr, Heinrich <<a href="mailto:grabmayr@biochem.mpg.de" class="">grabmayr@biochem.mpg.de</a>> wrote:</div>
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Hi Sebastien,</div>
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I am using bio-formats on an up-to-date Fiji with bio-formats 5.3 on OSX Sierra. I just checked and there are no new updates.</div>
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I’ll send you the sample Delta Vision file off list due to its file size.</div>
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Thanks</div>
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   Heinrich</div>
<br class="">
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<br class="">
<p class="airmail_on">On 24 February 2017 at 14:05:58, Sebastien Besson (Staff) (<a href="mailto:s.besson@dundee.ac.uk" class="">s.besson@dundee.ac.uk</a>) wrote:</p>
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<div class=""><span class="">Hi Heinrich,</span>
<div class=""><span class=""><br class="">
</span></div>
<div class="">
<div class=""><span class="">Your workflow looks reasonable and it is true that the metadata of the generated OME-TIFF is surprising.</span></div>
<div class=""><span class=""><br class="">
</span></div>
<div class=""><span class="">An immediate thought: which version of Bio-Formats are you using? From the schema used at export</span></div>
<div class=""><span class="">(2015-01), it looks like you are using BIo-Formats 5.1.x. Could you try upgrading to the latest version [1]</span></div>
<div class=""><span class="">and see if the problem persists?</span></div>
<div class=""><span class=""><br class="">
</span></div>
<div class=""><span class="">If this is still the case, we might need to have access to a sample file to try and reproduce the issue.</span></div>
<div class=""><span class=""><br class="">
</span></div>
<div class=""><span class="">Best,</span></div>
<div class=""><span class="">Sebastien</span></div>
<div class=""><span class=""><br class="">
</span></div>
<div class=""><span class=""> [1] <a href="http://downloads.openmicroscopy.org/bio-formats/" class="">http://downloads.openmicroscopy.org/bio-formats/</a></span></div>
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</span></div>
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</span>
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<div class=""><span class="">On 23 Feb 2017, at 15:21, Heinrich Grabmayr <<a href="mailto:h.grabmayr@physik.uni-muenchen.de" class="">h.grabmayr@physik.uni-muenchen.de</a>> wrote:</span></div>
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<span class="">Hi all,</span></div>
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<span class="">I am writing Python software that should be able to load data from various microscope manufacturers. I therefore went for the awesome OME / bioformats library. In order to access it from Python, I am using python-bioformats (<a href="https://github.com/CellProfiler/python-bioformats/tree/master/bioformats" class="">https://github.com/CellProfiler/python-bioformats/tree/master/bioformats</a>).</span></div>
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<span class="">Now I ran into the following issue: In some cases, I would like to first preprocess the files in ImageJ (e.g. extract one series). So I do that and save using Plugins->Bio Formats->Bio Formats Exporter, as an .ome.tiff.</span></div>
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<span class="">When I then load the file in my software, the number of slices in C and T are switched. Also, I can’t directly access metadata about channels. Apparently, the original metadata is encapsulated in a deeper layer. When I access the description
 of the first level of metadata, I get another omexml with approximately the original metadata - but it is rearranged in a way that makes me unable to access it the same way I access the original files (which are DeltaVision .dv files in this case). See below
 for example metadata of an original file and of a IJ-saved file.</span></div>
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<span class="">However, when I load the .ome.tiff into ImageJ, the Bio-formats importer reads the correct metadata, so it should be possible to let bioformats do the work, I suspect.</span></div>
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<span class="">Could anyone point me to a place where the Importer checks and finds the nested and correct metadata? Or tell me the solution?</span></div>
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<span class="">I have tried to look here</span></div>
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<span class=""><a href="https://github.com/openmicroscopy/bioformats/blob/develop/components/bio-formats-plugins/src/loci/plugins/in/ImporterMetadata.java" class="">https://github.com/openmicroscopy/bioformats/blob/develop/components/bio-formats-plugins/src/loci/plugins/in/ImporterMetadata.java</a></span></div>
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<span class="">and here</span></div>
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<span class=""><a href="https://github.com/openmicroscopy/bioformats/blob/develop/components/formats-bsd/utils/mipav/PlugInBioFormatsImporter.java" class="">https://github.com/openmicroscopy/bioformats/blob/develop/components/formats-bsd/utils/mipav/PlugInBioFormatsImporter.java</a></span></div>
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<span class="">, didn’t find anything suspicious, and am slightly overwhelmed with the size of the project.</span></div>
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<span class=""><br class="">
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<span class="">This is how python-bioformats gets metadata:</span></div>
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<span class=""><a href="https://github.com/CellProfiler/python-bioformats/blob/master/bioformats/formatreader.py#L992" class="">https://github.com/CellProfiler/python-bioformats/blob/master/bioformats/formatreader.py#L992</a></span></div>
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<span class="">Thanks very much</span></div>
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<span class="">    Heinrich</span></div>
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<span class="">————————————— Metadata of series 0 of original .dv(DeltaVision) file with 1 series————————————  </span></div>
<div class=""><span class=""><?xml version="1.0" encoding="UTF-8" standalone="no"?><OME xmlns="<a href="http://www.openmicroscopy.org/Schemas/OME/2015-01" class="">http://www.openmicroscopy.org/Schemas/OME/2015-01</a>" xmlns:xsi="<a href="http://www.w3.org/2001/XMLSchema-instance" class="">http://www.w3.org/2001/XMLSchema-instance</a>"
 xsi:schemaLocation="<a href="http://www.openmicroscopy.org/Schemas/OME/2015-01" class="">http://www.openmicroscopy.org/Schemas/OME/2015-01</a>
<a href="http://www.openmicroscopy.org/Schemas/OME/2015-01/ome.xsd" class="">http://www.openmicroscopy.org/Schemas/OME/2015-01/ome.xsd</a>"><Instrument ID="Instrument:0"><Objective Correction="PlanApo" ID="Objective:10612" Immersion="Oil" LensNA="1.42" Manufacturer="Olympus"
 Model="1-U2B933" NominalMagnification="60.0" WorkingDistance="150.0" WorkingDistanceUnit="µm"/></Instrument><Image ID="Image:0" Name="DeltaVision_Test.dv"><InstrumentRef ID="Instrument:0"/><ObjectiveSettings ID="Objective:10612"/><Pixels BigEndian="false"
 DimensionOrder="XYCZT" ID="Pixels:0" Interleaved="false" PhysicalSizeX="0.10829800367355347" PhysicalSizeXUnit="µm" PhysicalSizeY="0.10829800367355347" PhysicalSizeYUnit="µm" PhysicalSizeZ="0.20000000298023224" PhysicalSizeZUnit="µm" SignificantBits="16" SizeC="3"
 SizeT="1" SizeX="2048" SizeY="2048" SizeZ="1" Type="uint16"><Channel EmissionWavelength="676.0" EmissionWavelengthUnit="nm" ExcitationWavelength="632.0" ExcitationWavelengthUnit="nm" ID="Channel:0:0" NDFilter="0.0" SamplesPerPixel="1"><LightPath/></Channel><Channel
 EmissionWavelength="632.0" EmissionWavelengthUnit="nm" ExcitationWavelength="575.0" ExcitationWavelengthUnit="nm" ID="Channel:0:1" NDFilter="0.0" SamplesPerPixel="1"><LightPath/></Channel><Channel EmissionWavelength="523.0" EmissionWavelengthUnit="nm" ExcitationWavelength="475.0"
 ExcitationWavelengthUnit="nm" ID="Channel:0:2" NDFilter="0.0" SamplesPerPixel="1"><LightPath/></Channel><MetadataOnly/><Plane DeltaT="0.0" DeltaTUnit="s" ExposureTime="2.0" ExposureTimeUnit="s" PositionX="1688.268" PositionXUnit="reference frame" PositionY="-431.984"
 PositionYUnit="reference frame" PositionZ="0.0" PositionZUnit="reference frame" TheC="0" TheT="0" TheZ="0"/><Plane DeltaT="2.4860000610351562" DeltaTUnit="s" ExposureTime="5.0" ExposureTimeUnit="s" PositionX="1688.268" PositionXUnit="reference frame" PositionY="-431.984"
 PositionYUnit="reference frame" PositionZ="0.0" PositionZUnit="reference frame" TheC="1" TheT="0" TheZ="0"/><Plane DeltaT="7.808000087738037" DeltaTUnit="s" ExposureTime="10.0" ExposureTimeUnit="s" PositionX="1688.268" PositionXUnit="reference frame" PositionY="-431.984"
 PositionYUnit="reference frame" PositionZ="0.0" PositionZUnit="reference frame" TheC="2" TheT="0" TheZ="0"/></Pixels></Image><StructuredAnnotations xmlns="<a href="http://www.openmicroscopy.org/Schemas/SA/2015-01" class="">http://www.openmicroscopy.org/Schemas/SA/2015-01</a>"><XMLAnnotation
 ID="Annotation:0" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Wavelength 3 min. intensity</Key><Value>1641.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:1" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>DeltaVision_Test.dv Extended header Z0 W2 T0:photosensorReading</Key><Value>3809518.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:2" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>DeltaVision_Test.dv Extended header Z0 W0 T0:ndFilter</Key><Value>0.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:3" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>X axis angle</Key><Value>90.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:4" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>DeltaVision_Test.dv Extended header Z0 W0 T0:maxInten</Key><Value>7108.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:5" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>DeltaVision_Test.dv Extended header Z0 W1 T0:energyConvFactor</Key><Value>1.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:6" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Z axis tilt angle</Key><Value>0.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:7" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>DeltaVision_Test.dv Extended header Z0 W0 T0:minInten</Key><Value>247.0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:8" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Lens ID Number</Key><Value>10612</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:9" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Wavelength 4 (in nm)</Key><Value>0</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
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 ID="Annotation:11" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Column axis sequence</Key><Value>1</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
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<span class="">————————————— Metadata when opened with ImageJ saved using bio-formats exporter————————————  </span></div>
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 see the OME-TIFF web site: <a href="http://www.openmicroscopy.org/site/support/ome-model/ome-tiff/" class="">
http://www.openmicroscopy.org/site/support/ome-model/ome-tiff/</a>. --&gt;&lt;OME xmlns="<a href="http://www.openmicroscopy.org/Schemas/OME/2015-01" class="">http://www.openmicroscopy.org/Schemas/OME/2015-01</a>" xmlns:xsi="<a href="http://www.w3.org/2001/XMLSchema-instance" class="">http://www.w3.org/2001/XMLSchema-instance</a>"
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 BigEndian="false" DimensionOrder="XYCZT" ID="Pixels:0" Interleaved="false" PhysicalSizeX="0.10829800367355347" PhysicalSizeXUnit="µm" PhysicalSizeY="0.10829800367355347" PhysicalSizeYUnit="µm" PhysicalSizeZ="0.20000000298023224" PhysicalSizeZUnit="µm" SignificantBits="16"
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 NDFilter="0.0" SamplesPerPixel="1"&gt;&lt;LightPath/&gt;&lt;/Channel&gt;&lt;Channel EmissionWavelength="632.0" EmissionWavelengthUnit="nm" ExcitationWavelength="575.0" ExcitationWavelengthUnit="nm" ID="Channel:0:1" NDFilter="0.0" SamplesPerPixel="1"&gt;&lt;LightPath/&gt;&lt;/Channel&gt;&lt;Channel
 EmissionWavelength="523.0" EmissionWavelengthUnit="nm" ExcitationWavelength="475.0" ExcitationWavelengthUnit="nm" ID="Channel:0:2" NDFilter="0.0" SamplesPerPixel="1"&gt;&lt;LightPath/&gt;&lt;/Channel&gt;&lt;TiffData FirstC="0" FirstT="0" FirstZ="0" IFD="0"
 PlaneCount="1"&gt;&lt;UUID FileName="DeltaVisionTest_bioformatsIJexport.ome.tif"&gt;urn:uuid:6b04671a-1994-49ad-a7dc-9db604cab07d&lt;/UUID&gt;&lt;/TiffData&gt;&lt;TiffData FirstC="1" FirstT="0" FirstZ="0" IFD="1" PlaneCount="1"&gt;&lt;UUID FileName="DeltaVisionTest_bioformatsIJexport.ome.tif"&gt;urn:uuid:6b04671a-1994-49ad-a7dc-9db604cab07d&lt;/UUID&gt;&lt;/TiffData&gt;&lt;TiffData
 FirstC="2" FirstT="0" FirstZ="0" IFD="2" PlaneCount="1"&gt;&lt;UUID FileName="DeltaVisionTest_bioformatsIJexport.ome.tif"&gt;urn:uuid:6b04671a-1994-49ad-a7dc-9db604cab07d&lt;/UUID&gt;&lt;/TiffData&gt;&lt;Plane DeltaT="0.0" DeltaTUnit="s" ExposureTime="2.0"
 ExposureTimeUnit="s" PositionX="1688.268" PositionXUnit="reference frame" PositionY="-431.984" PositionYUnit="reference frame" PositionZ="0.0" PositionZUnit="reference frame" TheC="0" TheT="0" TheZ="0"/&gt;&lt;Plane DeltaT="2.4860000610351562" DeltaTUnit="s"
 ExposureTime="5.0" ExposureTimeUnit="s" PositionX="1688.268" PositionXUnit="reference frame" PositionY="-431.984" PositionYUnit="reference frame" PositionZ="0.0" PositionZUnit="reference frame" TheC="1" TheT="0" TheZ="0"/&gt;&lt;Plane DeltaT="7.808000087738037"
 DeltaTUnit="s" ExposureTime="10.0" ExposureTimeUnit="s" PositionX="1688.268" PositionXUnit="reference frame" PositionY="-431.984" PositionYUnit="reference frame" PositionZ="0.0" PositionZUnit="reference frame" TheC="2" TheT="0" TheZ="0"/&gt;&lt;/Pixels&gt;&lt;/Image&gt;&lt;/OME&gt;</Description><Pixels
 BigEndian="false" DimensionOrder="XYCZT" ID="Pixels:0" Interleaved="false" PhysicalSizeX="0.10829800426813266" PhysicalSizeXUnit="µm" PhysicalSizeY="0.10829800426813266" PhysicalSizeYUnit="µm" SignificantBits="16" SizeC="1" SizeT="3" SizeX="2048" SizeY="2048"
 SizeZ="1" Type="uint16"><Channel ID="Channel:0:0" SamplesPerPixel="1"><LightPath/></Channel><MetadataOnly/></Pixels></Image><StructuredAnnotations xmlns="<a href="http://www.openmicroscopy.org/Schemas/SA/2015-01" class="">http://www.openmicroscopy.org/Schemas/SA/2015-01</a>"><XMLAnnotation
 ID="Annotation:0" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>XResolution</Key><Value>92337</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:1" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>PhotometricInterpretation</Key><Value>Palette</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:2" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>PlanarConfiguration</Key><Value>Chunky</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:3" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>MetaDataPhotometricInterpretation</Key><Value>Monochrome</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:4" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>ImageLength</Key><Value>2048</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:5" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>BitsPerSample</Key><Value>16</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:6" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>ResolutionUnit</Key><Value>Centimeter</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:7" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>SamplesPerPixel</Key><Value>1</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:8" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Compression</Key><Value>Uncompressed</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:9" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>NumberOfChannels</Key><Value>3</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:10" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>Software</Key><Value>OME Bio-Formats</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation
 ID="Annotation:11" Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>MetaMorph</Key><Value>no</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation ID="Annotation:12"
 Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>ImageWidth</Key><Value>2048</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation ID="Annotation:13"
 Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>SampleFormat</Key><Value>unsigned integer</Value></OriginalMetadata></Value></XMLAnnotation><XMLAnnotation ID="Annotation:14"
 Namespace="<a href="http://openmicroscopy.org/OriginalMetadata" class="">openmicroscopy.org/OriginalMetadata</a>"><Value><OriginalMetadata><Key>YResolution</Key><Value>92337</Value></OriginalMetadata></Value></XMLAnnotation></StructuredAnnotations></OME></span></div>
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<span style="font-size:10pt;">The University of Dundee is a registered Scottish Charity, No: SC015096</span>
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