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<div>Hello </div>
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<div>I have now added the ability to import data from the table that Simon created, to PALM-siever.</div>
<div>Screenshots attached. Code in <a href="https://github.com/imunro/palm-siever">
https://github.com/imunro/palm-siever</a></div>
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<div> It’s currently quite slow, however.</div>
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<div>Best Wishes</div>
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<div>Ian </div>
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<div><img height="779" width="1211" apple-width="yes" apple-height="yes" apple-inline="yes" id="3B75F501-1150-4355-B790-2785374A5929" src="cid:717E872C-1C25-4566-9977-F5D07E458306@ic.ac.uk"></div>
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<div><img height="776" width="1211" apple-width="yes" apple-height="yes" apple-inline="yes" id="9D136D8A-3BB6-4EF2-89B6-C2DDF24768CD" src="cid:3C8BB36A-E833-4C47-8D91-C2C2EAF429CE@ic.ac.uk"></div>
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<div>On 26 Aug 2015, at 15:21, Ian Munro <<a href="mailto:i.munro@imperial.ac.uk">i.munro@imperial.ac.uk</a>> wrote:</div>
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Hi Seamus
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<div>If you want to try this there is quite a bit of code for interfacing matlab applications to OMERO in </div>
<div><a href="https://github.com/openmicroscopy/Imperial-FLIMfit/tree/OME51">https://github.com/openmicroscopy/Imperial-FLIMfit/tree/OME51</a>.</div>
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<div>Best Wishes</div>
<div><br>
</div>
<div>Ian</div>
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<div>On 26 Aug 2015, at 14:58, Seamus Holden <<a href="mailto:seamus.holden@gmail.com">seamus.holden@gmail.com</a>> wrote:</div>
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<div>For what it's worth, I'm happy to add this capability to PALMsiever too - not much work.<br>
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Best <br>
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Seamus<br>
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<div class="gmail_quote">
<div dir="ltr">On Tue, 25 Aug 2015 at 14:03 Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk">i.munro@imperial.ac.uk</a>> wrote:<br>
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<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div style="word-wrap:break-word">Hi Seamus
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</div>
<div>Not at all I’m happy to get any feedback I’m very much feeling my way here.</div>
<div>In the light of your comments and after discussion with the OME team we have moved on to Plan B. (or possibly E) which is to add to ThunderSTORM</div>
<div>the capability to load localisations from the data that Simon Li has generated.</div>
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<div>Best Wishes </div>
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<div>Ian</div>
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<div>On 24 Aug 2015, at 11:38, Seamus Holden <<a href="mailto:seamus.holden@gmail.com" target="_blank">seamus.holden@gmail.com</a>> wrote:</div>
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<div>Hi Ian<br>
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I should stress that although these comments are a bit negative I mean them only in a constructive fashion, and I am completely happy to be contradicted!<br>
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<div>There are already a couple of rendering tools available - mainly PALMsiever (which I helped develop, open source), ThunderSTORM (open-source) and VisP (closed source). Being academic software, all of them are a bit buggy, and slowly updated, but essentially,
the rendering bit is a (largely) solved problem, software wise. And regards the bugs/ missing features - I would suggest rather than develop new software, just ask for commit rights on PALMsiever or ThunderSTORM - they are both on GitHub!<br>
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The common localization format is on the other hand still an unsolved problem.The nicest point to my mind about focussing just on this aspect is that it's a small(-ish), well defined problem, which it would be quite useful to solve.
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<div>Best wishes<br>
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Seamus<br>
<p class="MsoNormal"><a name="msg-f:1510481925494119892__MailAutoSig"><span style="color:#1f497d">Dr Seamus Holden</span></a></p>
<p class="MsoNormal"><span><span style="color:#1f497d">University Research Fellow</span></span></p>
<div><span><span style="color:#1f497d"> </span></span><br>
</div>
<p class="MsoNormal"><span><span style="color:#1f497d">Centre for Bacterial Cell Biology</span></span></p>
<p class="MsoNormal"><span><span style="color:#1f497d">Baddiley-Clark Building</span></span></p>
<p class="MsoNormal"><span><span style="color:#1f497d">Newcastle University</span></span></p>
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<p class="MsoNormal"><span><span style="color:#1f497d">Newcastle upon Tyne</span></span></p>
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<div><span><span style="color:#1f497d"> </span></span><br>
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<p class="MsoNormal"><span><span style="color:#1f497d">Phone: +44 (0)191 208 3230</span></span></p>
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<div dir="ltr">On Mon, 24 Aug 2015 at 10:09 Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk" target="_blank">i.munro@imperial.ac.uk</a>> wrote:<br>
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Hello All<br>
<br>
Does anyone have any comments on Kwasi’s document?.<br>
Based on his thoughts I’ve started looking at developing an open-source OMERO-capable<br>
reconstruction/rendering tool, although not full-time I fear, so progress is slow.<br>
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If anyone has any thoughts it would be good to hear them at this early stage.<br>
<br>
Thanks<br>
<br>
Ian Munro<br>
<br>
Photonics Department<br>
Imperial College, London.<br>
<br>
On 7 Aug 2015, at 13:43, Kwasi Amoako Kwakwa <<a href="mailto:k.kwakwa@imperial.ac.uk" target="_blank">k.kwakwa@imperial.ac.uk</a>> wrote:<br>
<br>
> Hello All,<br>
><br>
> I am a postdoc in Paul French's group at Imperial working on<br>
> STORM/localization microscopy. I've been talking to Ian Munro a lot trying to<br>
> hash out what I think are the outstanding problems with storing and viewing<br>
> localization data and I thought it might be a good idea to share what I have<br>
> so far and see if people agree and if you have any suggestions or things to<br>
> add.<br>
><br>
> Thanks,<br>
> Kwasi Kwakwa<br>
><br>
><br>
> --<br>
> Kwasi A. Kwakwa<br>
> Research Associate, Photonics Department<br>
> Imperial College London<Localization Microscopy Issues.docx>_______________________________________________<br>
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