<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;">
<div>Hi,</div>
<div><br>
</div>
<div>The following page lists the supported metadata fields for the flex format,</div>
<div><a href="http://www.openmicroscopy.org/site/support/bio-formats4/formats/evotecperkinelmer-opera-flex-metadata.html">http://www.openmicroscopy.org/site/support/bio-formats4/formats/evotecperkinelmer-opera-flex-metadata.html</a></div>
<div><br>
</div>
<div>It would be great if you could upload a sample set (images from one or two wells) to , </div>
<div><a href="http://qa.openmicroscopy.org.uk/qa/upload/?">http://qa.openmicroscopy.org.uk/qa/upload/?</a></div>
<div><br>
</div>
<div>This will allow us to check the files locally and suggest suitable API calls.</div>
<div><br>
</div>
<div>Best,</div>
<div>Balaji</div>
<div><br>
</div>
<span id="OLK_SRC_BODY_SECTION">
<div style="font-family:Calibri; font-size:11pt; text-align:left; color:black; BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 0in; PADDING-RIGHT: 0in; BORDER-TOP: #b5c4df 1pt solid; BORDER-RIGHT: medium none; PADDING-TOP: 3pt">
<span style="font-weight:bold">From: </span><Bonamy>, Ghislain <<a href="mailto:ghislain.bonamy@novartis.com">ghislain.bonamy@novartis.com</a>><br>
<span style="font-weight:bold">Date: </span>Monday, 17 March 2014 11:27<br>
<span style="font-weight:bold">To: </span>"<a href="mailto:ome-devel@lists.openmicroscopy.org.uk">ome-devel@lists.openmicroscopy.org.uk</a>" <<a href="mailto:ome-devel@lists.openmicroscopy.org.uk">ome-devel@lists.openmicroscopy.org.uk</a>><br>
<span style="font-weight:bold">Subject: </span>[ome-devel] Opera Flex file lattest changes<br>
</div>
<div><br>
</div>
<div xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<meta name="Generator" content="Microsoft Word 14 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Consolas;
panose-1:2 11 6 9 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri","sans-serif";}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
{mso-style-priority:34;
margin-top:0in;
margin-right:0in;
margin-bottom:0in;
margin-left:.5in;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:"Calibri","sans-serif";}
span.EmailStyle17
{mso-style-type:personal-compose;
font-family:"Calibri","sans-serif";
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-family:"Calibri","sans-serif";}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
/* List Definitions */
@list l0
{mso-list-id:1273047712;
mso-list-type:hybrid;
mso-list-template-ids:1352151110 67698705 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
@list l0:level1
{mso-level-text:"%1\)";
mso-level-tab-stop:none;
mso-level-number-position:left;
text-indent:-.25in;}
@list l0:level2
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-number-position:left;
text-indent:-.25in;}
@list l0:level3
{mso-level-number-format:roman-lower;
mso-level-tab-stop:none;
mso-level-number-position:right;
text-indent:-9.0pt;}
@list l0:level4
{mso-level-tab-stop:none;
mso-level-number-position:left;
text-indent:-.25in;}
@list l0:level5
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-number-position:left;
text-indent:-.25in;}
@list l0:level6
{mso-level-number-format:roman-lower;
mso-level-tab-stop:none;
mso-level-number-position:right;
text-indent:-9.0pt;}
@list l0:level7
{mso-level-tab-stop:none;
mso-level-number-position:left;
text-indent:-.25in;}
@list l0:level8
{mso-level-number-format:alpha-lower;
mso-level-tab-stop:none;
mso-level-number-position:left;
text-indent:-.25in;}
@list l0:level9
{mso-level-number-format:roman-lower;
mso-level-tab-stop:none;
mso-level-number-position:right;
text-indent:-9.0pt;}
ol
{margin-bottom:0in;}
ul
{margin-bottom:0in;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
<div lang="EN-US" link="blue" vlink="purple">
<div class="WordSection1">
<p class="MsoNormal">Hi everyone,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">It has been a while since I last posted. I had a quick question, which Melissa can probably answer. The flex file form changed slightly in v2.0 of Evoshell. In particular the file structure. The microscope now generates the files as 1 file
per field (as opposed to one file per image). As before the rest of the dimensions (ie. Z-stacks and channels) are encoded into the same file.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I was wondering how I could request the metadata for a full well in s ingle call? In particular I would like to make our webservice that retrieves the images compatible with the previous version with as little changes as possible. If there
was a way to do things like:<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span style="mso-list:Ignore">1)<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman';">
</span></span><!--[endif]-->Get the number of fields for Well B01 (002001*)<o:p></o:p></p>
<p class="MsoListParagraph" style="text-indent:-.25in;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span style="mso-list:Ignore">2)<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman';">
</span></span><!--[endif]-->Get the image slice for WellB01 Channel2, Z-stack <o:p>
</o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">That would be perfect.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks in advance for the help. Hopefully this is clear, otherwise I can post more details.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">Ghislain Bonamy, PhD<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">__________________________________________<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">Group Leader<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">Novartis<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">Institute for<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">Tropical<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family: Arial, sans-serif;">Diseases<o:p></o:p></span></p>
</div>
</div>
</div>
</span><br>
<span style="font-size:10pt;">The University of Dundee is a registered Scottish Charity, No: SC015096</span>
</body>
</html>