Hi Andrew,<br><br><br><blockquote style="margin:0pt 0pt 0pt 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex" class="gmail_quote">
We are in the process of trying to resolve some outstanding differences
between the OME Schema and the way this is represented in the OMERO
Database.<br><br>
One of the differences is how MicrobeamManipulations are linked to Images.<br><br>
We are looking for some real use cases. Can anyone who is working with
MicrobeamManipulation, either in code against OMERO or by writing
OME-XML or OME-TIFF files please get in touch.<br></blockquote>
<br>When we first added MicrobeamManipulation to the schema, LOCI had some use cases for it: ablation and photobleaching. At the time we had recently added a second laser to our main optical workstation, which provided an ablation capability. So our main requirement was that we could record all such ablation events in OME-XML, with a timestamp of when the event occurred, as well as the center point of the event. These events would accrue over time during acquisition based on manual user activation, and ultimately get written out to the final OME-XML block at the end of the run.<br>
<br>Unfortunately, my understanding is that due to a technical issue, we had to remove the ablation laser from our system, and it is not currently available, so WiscScan does not yet support recording of MicrobeamManipulation events into the metadata.<br>
<br>That said, we do hope to provide such capabilities again in the future, so continued support of MicrobeamManipulation elements is appreciated.<br><br>Regards,<br>Curtis<br><br><br><div class="gmail_quote">On Mon, Apr 2, 2012 at 5:20 AM, Andrew Patterson <span dir="ltr"><<a href="mailto:ajpatterson@lifesci.dundee.ac.uk" target="_blank">ajpatterson@lifesci.dundee.ac.uk</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hello People,<br>
<br>
We are in the process of trying to resolve some outstanding differences between the OME Schema and the way this is represented in the OMERO Database.<br>
<br>
One of the differences is how MicrobeamManipulations are linked to Images.<br>
<br>
We are looking for some real use cases. Can anyone who is working with MicrobeamManipulation, either in code against OMERO or by writing OME-XML or OME-TIFF files please get in touch.<br>
<br>
Either reply to the lists or contact me directly:<br>
<a href="mailto:ajpatterson@lifesci.dundee.ac.uk" target="_blank">ajpatterson@lifesci.dundee.ac.uk</a><br>
<br>
Thanks for your continued support,<br>
<br>
Andrew<br>
<br>
--<br>
Andrew Patterson<br>
Software Developer, Open Microscopy Environment<br>
Wellcome Trust Centre for Gene Regulation and Expression<br>
University of Dundee<br>
<br>
<br>
<br>
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</blockquote></div><br>