<div class="gmail_quote">Hi Nick,<br><br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">Of
perhaps more importance: does OME care about IFDs? Are there required
IFD data? Or does the completeness of my IFDs only make the file more
compatible with TIFF readers and not really effect OME? Like, could I
just skip the details in the TIFF IFDs and just have things like
ImageWidth, ImageLength, etc, which I'm sure the bio-formats tools need
to correctly parse the data?</blockquote><div><br>OME does not specifically care about any IFD values, no -- at least, not any above and beyond what would normally be required for a regular TIFF. You should populate exactly what you would normally populate if the file were not OME-TIFF. Any overlap between OME metadata and TIFF IFD values (SizeX vs. ImageWidth, SizeY vs. ImageHeight, etc.) should be consistent -- if it is not, Bio-Formats will issue a warning message and the resultant behavior may not be what you want.<br>
</div></div><br>-Curtis<br><br><div class="gmail_quote">On Tue, Sep 8, 2009 at 5:48 AM, Nick Perry <span dir="ltr"><<a href="mailto:nperry@stanford.edu">nperry@stanford.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
To expand on my question:<br><br>If I have a lifetime channel in my image, and I want to put it in a TIFF file format, then the IFD for that lifetime channel plane at a given Z, Time could have:<br><br>BitsPerSample = 16 (if each 'pixel' in this plane should be read as a SHORT)<br>
PhotometricInterpretation = 1<br>SamplesPerPixel = 1<br><br>Is this about right? <br><br>Of perhaps more importance: does OME care about IFDs? Are there required IFD data? Or does the completeness of my IFDs only make the file more compatible with TIFF readers and not really effect OME? Like, could I just skip the details in the TIFF IFDs and just have things like ImageWidth, ImageLength, etc, which I'm sure the bio-formats tools need to correctly parse the data?<div>
<div></div><div class="h5"><br>
<br><div class="gmail_quote">On Tue, Sep 8, 2009 at 9:49 AM, Nick Perry <span dir="ltr"><<a href="mailto:nperry@stanford.edu" target="_blank">nperry@stanford.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
In creating an OME-TIFF file, are there specific TIFF IFD fields that are "standard" to use in OME-TIFF files? Which are they? Is there any sort of documentation available for this?<br><br>Thanks,<br><font color="#888888">Nick<br>
</font></blockquote></div><br>
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