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<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'>Harry,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'>Thanks for your interest in our work. <o:p></o:p></span></font></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Bernd:<o:p></o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>Interesting stuff: some comments below.<o:p></o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>On Nov 23, 2005, at 2:21 PM, Bernd Jagla wrote:<o:p></o:p></span></font></p>
</div>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">
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<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Then we want
to display a particular feature for all the wells/plates as a heat map to
identify by eye any biases or problems in the data set. For this we envision
right now using Partek’s Screener Solution which could interface with OME
through ODBC. A simple (complex) select statement should bring all the
necessary information into the Screener Solution.</span></font><o:p></o:p></p>
</div>
</blockquote>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>As a data visualization guy, I'm interested in what you're doing here -
can you provide more detail?<o:p></o:p></span></font></p>
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<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>When you say heat map, I assume that the feature for the wells/plates
would be something that has a numeric value? Do you want to do dendrograms, or
is outlier detection your main task?<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>At the moment I am just looking for
plotting the numerical values we came up with from analyzing our images. So the
focus for me is data integration and getting things going. We don’t even
have any valuable data yet. I will keep you informed of where we are going…<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<br>
<o:p></o:p></span></font></p>
<div>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><O:P></O:P><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">Then
we have to select hits and analyze the screen: <O:P></O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Display a particular feature for all wells and plates. (This
is for quality control). <O:P></O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Select a relevant feature and a threshold.<O:P></O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Write back the results (somehow) to OME. Those results will
eventually group the results in different categories (hit, non-hit, not-known,
positive control, negative control, etc). This actually still has to be solved.</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE>Custom
STs, or CategoryGroup/Category annotations can help. <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Do you mean we should generate an ST for
each group, or bypass the given dataset structure? I will have to look into the
CategoryGroup/Category annotations a bit more.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<br>
<o:p></o:p></span></font></p>
<div>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><O:P></O:P><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">Then
we want to see/ cross-link the results with other screens: of these hits, which
other assays where they active in? Which compounds where active in a given set
of screens? Give me a complete profile of all the compounds and in which assays
they where active, given a specified threshold etc.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>when you say hit, do you mean image? ie, which other
datasets/experiments is a given image in? Also, can you clarify threshold?<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 color=navy face="Times New Roman"><span
style='font-size:12.0pt;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>A hit is defined by a value derived from
an image being higher than a predefined threshold. The threshold is usually set
such that we get a certain number of hits.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Images are confocal microscope images of cell-based
assays that were treated with different compounds.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<br>
<o:p></o:p></span></font></p>
<div>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><O:P></O:P><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">Of
course at some point we want to see the compounds with the results and
everything but this will wait a while and needs probably further discussions on
how to integrate all this.</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE>right.<br>
<br>
<o:p></o:p></span></font></p>
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<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><O:P></O:P><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">Then
we have to push some of the data to PubChem at the NIH. How could we do this? A
simple script with select statements could do the work, no? Maybe we can write
a chain module that automatically propagates the data to PubChem (they use XML
– their own format of course).<O:P></O:P></span></font><o:p></o:p></p>
</div>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt'>
<div>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><O:P><br>
<br>
</O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address"></span></font><o:p></o:p></p>
</div>
</blockquote>
</O:P>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
</div>
</O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>One possibility would be to use OME's exporting facilities to generate
OME XML files and then XSLT to translate into PubChem's schema.<o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><br>
<font color=navy><span style='color:navy'>I am actually having problems with
exporting my images and information, but I have to admit that I didn’t do
much about it yet. I just tried the export image function which didn’t
work.</span></font><o:p></o:p></span></font></p>
<div>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;
text-indent:.5in'><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><O:P><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address"> </O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>At the
moment I believe that the most important part is to improve (or for me to learn
how to do) the grouping features of OME. I don’t think it is good that
you have to know in advance that you want to add something to a data set. From
my experience I would rather play with the data and mark e.g. bad wells along
the way, select images that look interesting in one way or the other and say:
Wow they look cool lets make a new dataset and collect them here, or lets add
them to the list of interesting data. As far as I know this is only possible
once you know the name of the data set, and then you have to search for them
again.<O:P></O:P></span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE>there
are lots of ways to think about this sort of functionality - we've discussed
pieces of it. The closest thing that is implemented is the category
group/category annotation tool, but that does require starting from a selected
set of images.<o:p></o:p></span></font></p>
</div>
<div>
<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">
<div>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><O:P> </O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Also for the
sake of better understanding can you please point me to all the relevant
information concerning data sets: how to programmatically do it, how to do it
with ODBC, and the web interface?</span></font><o:p></o:p></p>
</div>
</blockquote>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE>The
best places to start from are in the "Developer's documentation"
section of the web site links. We can give more feedback for more
specific questions.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Ok, I will try to start there (after I
have read in the results correctly).<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Thanks a lot,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Bernd<o:p></o:p></span></font></p>
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<blockquote style='margin-top:5.0pt;margin-bottom:5.0pt' type=cite><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceType"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PlaceName"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="Street"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="PostalCode"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="State"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><O:SMARTTAGTYPE namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="address">
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<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><O:P> </O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>I would be
more than happy to explain in more detail or discuss our strategies as we are
just in the process of getting started…<O:P></O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><O:P> </O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Thanks a lot
for all your kind help.<O:P></O:P></span></font><o:p></o:p></p>
<p class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt:auto'><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'><O:P><br>
<br>
</span></font><o:p></o:p></p>
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</blockquote>
</O:P>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE></O:SMARTTAGTYPE>You're
very welcome...<o:p></o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'>-harry<o:p></o:p></span></font></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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