[ome-devel] ADF images of BD Pathway Bioimager

Jason H qz8w9l at gmail.com
Thu Apr 29 19:40:47 BST 2010


> The raw microscopy information is stored in a proprietary file, which is the
> adf file. BD Pathway has not shared a specification for the adf file, and it
> is subject to change at the whim of the BD. The Experiment.exp file that is
> exported from this is a text file in a known format.

Hi Shawn,

Thanks a lot for the info.

> Support for the adf file could be added, but it would just use it to locate
> the experiment.exp file in the same directory.

Then why are there as many .adf files as the number of dyes used in a
scanning run
in each well directory?  For a 96-well experiment with 3 dyes, we have
288 .adf files
of varying byte sizes.  If one single experiment.exp file serves the
purpose, why
bother with so many useless files scattering around?  Just thinking out loud.

My real purpose to see what's inside of a .adf file is to look for a
"stencil" file for each
dye.  The attached stencil.png is an example of what I mean by a
stencil file and
cells.png is the original image from which stencil.png is derived.

Best wishes,

Jason
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