<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear All-<div><br></div><div>The following seminar will be of interest to anyone using microscopy on live cells. Bob Clegg is one of the founders of FLIM and has continuously been involved in developing new advances and applications in FLIM<div><br></div><div><div><br></div><div>Dr Bob Clegg, University of Illinois, Urbana-Champaign, USA, will give a seminar in the WTB Seminar Rooms at 2.00 pm <br><br>"Fluorescence lifetime imaging (FLI) - How it helps us excavate the <br>rugged multi-spectral fluorescence terrain of complex biological <br>systems."<br><br>Host: David Lilley<br><br>All welcome<br><br>Abstract:<br>Fluorescence is a very powerful method for sensitive and detailed <br>analysis of molecular mechanisms. One of the primary goals of physical <br>molecular biology is to understand biological mechanisms on the <br>molecular scale, and fluorescence imaging is a powerful technique for <br>analyzing cellular biological systems. However, in a complex <br>environment of a biological cell, or living tissue, the situation <br>rapidly becomes exponentially more difficult than in a simple <br>cuvette-based system to derive quantitative data. These difficulties <br>pose a fascinating challenge for fluorescence analysis. This talk will <br>describe fluorescence lifetime imaging, which is a method of acquiring <br>fluorescence lifetimes at every pixel of a fluorescence image. A short <br>introduction will describe our instrumentation and review the major <br>reasons why we are interested in fluorescence lifetimes what type of <br>information is available from the fluorescence lifetimes. Then several <br>applications of FLI will be presented, including studies of <br>photosynthesis using the live algae Chlamydomonas, diagnosis of <br>prostate cancer biopsies, studies of ALA instigated protoporphyrin IX <br>production in living cells, pH variation across human skin, and recent <br>results on a new redox sensor. In addition, some new methods of <br>analysis will be presented that increase dramatically the ability for <br>analyzing FLI data from complex living systems.<br><div><br class="webkit-block-placeholder"></div><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><div style="font-family: Helvetica; ">Cheers,</div><div style="font-family: Helvetica; "><br></div><div style="font-family: Helvetica; ">Jason</div><div style="font-family: Helvetica; "><br class="Apple-interchange-newline"><br class="khtml-block-placeholder"></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">**************************</span></div><div style="font-family: Helvetica; "><font class="Apple-style-span" face="Arial">Wellcome Trust Centre for Gene Regulation & Expression</font></div><div style="font-family: Helvetica; "><font class="Apple-style-span" face="Arial">College of Life Sciences</font></div><div style="font-family: Helvetica; "><font class="Apple-style-span" face="Arial">MSI/WTB/JBC Complex</font></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">University of Dundee</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Dow Street</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Dundee DD1 5EH</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">United Kingdom</span></div><div style="font-family: Helvetica; "><br style="font-family: Helvetica; "></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">phone (01382) 385819</span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Intl phone: 44 1382 385819 </span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">FAX (01382) 388072 </span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">email: <a href="mailto:jason@lifesci.dundee.ac.uk">jason@lifesci.dundee.ac.uk</a></span></div><div style="font-family: Helvetica; "><br style="font-family: Helvetica; "></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Lab Page: <a href="http://www.dundee.ac.uk/lifesciences/swedlow/">http://www.dundee.ac.uk/lifesciences/swedlow/</a></span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">Open Microscopy Environment: <a href="http://openmicroscopy.org">http://openmicroscopy.org</a></span></div><div style="font-family: Helvetica; "><span class="Apple-style-span" style="font-family: Helvetica; ">**************************</span></div><div style="font-family: Helvetica; "><br class="webkit-block-placeholder"></div><div style="font-family: Helvetica; "><div>The University of Dundee is a Scottish Registered Charity, No. SC015096.</div></div><br class="Apple-interchange-newline"></span><br class="Apple-interchange-newline"> </div><br></div></div></div></body></html>