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<p class="MsoNormal">Dear Ali,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Sorry for the slow reply! You can load both channels by going to File>Load Polarisation Resolved Data…<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">There have been some improvements in the anisotropy analysis in the new version which will be released soon. You can try it out here:<o:p></o:p></p>
<p class="MsoNormal"><a href="https://storage.cloud.google.com/flimfit-downloads/latest/5.1.1-36-gd811f59d/FLIMfit%205.1.1-36-gd811f59d%20Setup%20x64.exe">https://storage.cloud.google.com/flimfit-downloads/latest/5.1.1-36-gd811f59d/FLIMfit%205.1.1-36-gd811f59d%20Setup%20x64.exe</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Let me know if you have any more problems<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks<o:p></o:p></p>
<p class="MsoNormal">Sean<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> FLIMfit-users <flimfit-users-bounces@lists.openmicroscopy.org.uk>
<b>On Behalf Of </b>Support list for users of FLIMfit Fluorescence Lifetime Imaging tool. via FLIMfit-users<br>
<b>Sent:</b> 08 July 2019 09:42<br>
<b>To:</b> flimfit-users@lists.openmicroscopy.org.uk<br>
<b>Subject:</b> [FLIMfit-users] Importing .sdt file for Anisotropy analysis<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="DE">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE"><o:p> </o:p></span></p>
<p class="MsoNormal">I would like to analyses my time-resolved polarization data with FLIMfit 5.1.1. The data is acquired with two B&H SPC150 modules each connected to a HPM detector.
<o:p></o:p></p>
<p class="MsoNormal">The point is that each time I load the .sdt data, only one of the channel can be loaded. Would anybody know hot properly load data for anisotropy analysis?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">All the best,<o:p></o:p></p>
<p class="MsoNormal">Ali<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:#767171">--
</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Dr. Dr. rer. nat.</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Imaging Specialist</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Light Microscopy Facility</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Technische Universität Dresden</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Center for Molecular and Cellular Bioengineering (CMCB)</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">DFG-Center for Regenerative Therapies Dresden</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Cluster of Excellence</span><span style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Fetscherstr. 105</span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">01307 Dresden</span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Tel: +49 (0)351-463-40230</span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Fax: +49 (0)351-463-40038</span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:gray">Webpage:
</span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><a href="http://www.crt-dresden.de/"><span style="color:#3366FF">www.crt-dresden.de</span></a>,</span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:#3366FF">
<a href="http://www.biodip.de/"><span style="color:#3366FF">www.biodip.de</span></a></span><span lang="DE" style="font-size:9.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
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