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</head>
<body lang="EN-AU" link="blue" vlink="purple">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">We are pleased to announce the release of FLIMfit<i>
</i>5.0.3, available for download at <span style="color:#0070C0"><a href="http://flimfit.org/downloads/latest"><span style="color:#0070C0">http://flimfit.org/downloads/latest</span></a>.</span><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Please note that we have updated to Matlab Runtime 2016b, Mac users will need to install the new runtime, available from the downloads page.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">A guide to using FLIMfit to analyse multiphoton FLIM data with tutorial videos is available in a recent paper,
<span style="color:#0070C0"><a href="https://doi.org/10.1016/j.ymeth.2017.04.014"><span style="color:#0070C0">Conway, JRW, Warren, SC & Timpson, P, Methods (2017)</span></a></span><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">New features<o:p></o:p></span></b></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l13 level1 lfo10">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">New segmentation manager with new freehand tool options and improved performance.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l13 level1 lfo10">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Ability to estimate IRF from a mono-exponential reference using an exponentially modified gaussian model. This can produce a high quality IRF for TCSPC detectors
without secondary peaks or significant afterpulsing.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l13 level1 lfo10">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Add support for Picoquant HydraHarp and TimeHarp data files.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l13 level1 lfo10">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Add support for data acquired during bidirectional scan.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l13 level1 lfo10">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Where possible determine repetition rate from data (currently ptu/pt3 files).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Performance Enhancements<o:p></o:p></span></b></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l8 level1 lfo14">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Significantly improved loading times for some data formats.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l8 level1 lfo14">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Added option to display smoothed data in the decay tab. When off, this significantly reduces the time to switch between datasets.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l8 level1 lfo14">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">This option does not affect smoothing during data fitting.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l8 level1 lfo14">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Improved performance selecting/deselecting images to fit.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l8 level1 lfo14">
<![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Update to Bioformats 5.4.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Other Enhancements<o:p></o:p></span></b></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l6 level1 lfo6"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Add option to plot weighted or unweighted values in plotter.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l6 level1 lfo6"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Visual enhacements to plotter.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l6 level1 lfo6"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Enable plotting by image number.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l6 level1 lfo6"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Allow setting default Gate Max value.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l6 level1 lfo6"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Improved support for older MacOS X versions (10.9.x).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Bug fixes<o:p></o:p></span></b></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l3 level1 lfo3"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Allow t0 shift map estimation from any loaded dataset (rather than first only)<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l3 level1 lfo3"><![if !supportLists]><span style="font-size:10.0pt;font-family:Symbol"><span style="mso-list:Ignore">·<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Fix crash when fitting t0 and offset locally<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Kind Regards,<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;line-height:14.0pt;background:white">
<span style="font-size:10.0pt;font-family:"Arial","sans-serif"">Dr Sean Warren, Garvan Institute of Medical Research<br>
Dr Ian Munro, Photonics Group, Imperial College, London<o:p></o:p></span></p>
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<span style="font-size:10.0pt;font-family:"Arial","sans-serif""><o:p> </o:p></span></p>
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<b><span style="font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-language:EN-AU">Dr. Sean Warren </span></b><span style="font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-language:EN-AU">| Research Officer<br>
Invasion and Metastasis<br>
Cancer Division<br>
<b>Garvan Institute of Medical Research </b><br>
The Kinghorn Cancer Centre,<b> </b>370 Victoria Street, Darlinghurst, NSW 2010</span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";mso-fareast-language:EN-AU"><o:p></o:p></span></p>
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