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Hi Susana 
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<div class="">Did any of that help at all?</div>
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<div class="">Best Wishes</div>
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<div class="">Ian </div>
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<div class="">On 16 Apr 2016, at 10:42, Munro, Ian <<a href="mailto:i.munro@imperial.ac.uk" class="">i.munro@imperial.ac.uk</a>> wrote:</div>
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<div class="">Dear Susana<br class="">
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I’m afraid it’s not clear to me  why this is so time-consuming so the following may not be of much help.<br class="">
However a few things that may be of use are:<br class="">
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Are you aware that the Bio-Formats Matlab Toolbox allows you to write ome-tiffs directly from Matlab?<br class="">
See <a href="https://downloads.openmicroscopy.org/bio-formats/5.1.8/" class="">https://downloads.openmicroscopy.org/bio-formats/5.1.8/</a> and we can provide example code if needed/<br class="">
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FLIMfit  can load multiple files using the File-> Load FLIM Dataset menu item and process them all <br class="">
in the same way so you could generate a large number of ome-tiffs and then process them in a single step.<br class="">
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Also, please be aware that the source code is available from <a href="https://github.com/imperial-photonics/FLIMfit" class="">https://github.com/imperial-photonics/FLIMfit</a><br class="">
so you could, in theory simply call the c++ back end from matlab.<br class="">
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There is an example file at <a href="https://github.com/imperial-photonics/FLIMfit/blob/master/FLIMfitFrontEnd/minimal_example.m" class="">https://github.com/imperial-photonics/FLIMfit/blob/master/FLIMfitFrontEnd/minimal_example.m</a><br class="">
but I’m not sure how recently that has been updated so it may well need some work.<br class="">
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Best Wishes <br class="">
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Ian <br class="">
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