[FLIMfit-users] Workflow to load SVB and TVB

[Munro, Ian]

Hi Romain

As far as I know it’s just Background > Load Time-varying background. 
Both Sean and  Hugh are more familiar with this area though.

Ian


> On 24 Aug 2016, at 11:39, R.F. Laine <rfl30 at cam.ac.uk> wrote:
> 
> Dear all,
> I have acquired a set of data which include:
> - a dark background set of images (laser off)
> - a TVB with laser on, but with no fluorescent samples present
> - my datasets
> 
> I have noticed that there is some TVB so I cannot ignore it.
> I would like to be able to load both my spatially varying background from the dark background and also load the TVB properly.
> The SVB works is loaded fine when using Background > Load time-averaged Background...
> 
> It is more complicated for the TVB.
> Should I just load the tiff using Background > Load Time-varying background... or open the dataset separately and create TVB intensity map?
> What is the difference?
> 
> The documentation
> http://docs.flimfit.org/introduction.html?highlight=tvb
> seems to imply it is not possible to load both TVB and SVB.
> 
> Many thanks for your help.
> 
> Best regards,
> 
> Romain
> 
> 
> -- 
> Dr. Romain Laine, PhD in Biophotonics
> Laser Analytics Group
> Department of Chemical Engineering and Biotechnology
> University of Cambridge
> New Museums Site
> Pembroke Street, Cambridge, CB2 3RA, UK
> T: (+44)1223330133