[FLIMfit-users] Fwd: FLIMfit is not fitting B-H data nor PQ data

Munro, Ian i.munro at imperial.ac.uk
Fri Sep 19 13:30:01 BST 2014




Hi Paul

You can drag the limits of the text boxes if you have a big enough monitor  but perhaps we should adjust the defaults.
Anyway  the answer is ‘Display’ and ‘Merged’.

Ticking Display  causes the false-colour Flim map of the associated fit parameter to be displayed (with the chosen limits).
Merged shows the same Colour map but weighted with the intensity of the original  data.
This emphasises the brighter (& therefore less noisy) areas & also allows you to look at the Lifetime and structural information in a single image.

All the Best

Ian

On 19 Sep 2014, at 12:53, Paul Thomas (SCI) <P.Thomas at uea.ac.uk<mailto:P.Thomas at uea.ac.uk>> wrote:

Hi Ian,

Sorry, but in the Images tab what are “Dis...” and “Me...”?

Thanks,
Paul.
______________________________________
Dr. Paul Thomas, Manager,
The Henry Wellcome Laboratory for Cell Imaging,
Faculty of Science,
University of East Anglia,
Norwich Research Park,
Norwich,
NR4 7TJ,
United Kingdom.
e-mail: p.thomas at uea.ac.uk<mailto:p.thomas at uea.ac.uk>
Tel: +44-1603-592196
Fax: +44-1603-592250
Imaging web-site: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab
Personal web-page: https://www.uea.ac.uk/biological-sciences/research/facilities/henry-wellcome-lab/people


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